![]() The various steps in the preparation of SLC-15 with stallion semen. However, in some cases (particularly for boar semen), it would be convenient to be able to process even larger volumes of semen per tube to avoid using more than one centrifuge or processing tubes in more than one centrifuge cycle. also reported a method for SLC in 200 mL tubes for stallion semen although these tubes do not fit the types of centrifuge commonly found on studs. The SLC method has been scaled-up successfully with stallion semen from 12 mL tubes into 50 mL tubes (Figure 2), whereas the method for boar semen has been scaled-up into 50 mL and 100 mL tubes without apparent loss of sperm quality. ![]() Even scaling up to 4.5 mL extended semen in 12 mL tubes is only feasible for a small number of ejaculates (Figure 1). Therefore, it would not be practical to use the original SLC method in the field to prepare spermatozoa for AI, since approximately 100–300 tubes each processing 1.5 mL extended semen would be needed to produce the requisite number of spermatozoa for equine or porcine AI. Ī major obstacle to the use of SLC to process stallion or boar spermatozoa for (AI) is the large volume of the ejaculate and the relatively high sperm number in the insemination dose. ![]() Species-specific formulations of Androcoll have been developed for stallion, boar, bull, dog, and cat. Since morphology and chromatin integrity have been linked to pregnancy rates in mares following AI, it has been postulated that the improvement in sperm quality seen following SLC could be reflected in improved pregnancy rates following (AI) if other factors (such as timing of insemination relative to ovulation and the skill of the inseminator) are optimal. ![]() In addition to selecting the most robust spermatozoa, SLC also removes them from seminal plasma. Capacitation and the acrosome reaction are not affected by SLC in stallion spermatozoa. Single Layer centrifugation (SLC) through a colloid has been advocated as a biomimetic method for improving sperm quality in artificial insemination (AI) doses, since it selects morphologically normal spermatozoa with intact membranes and good chromatin integrity in a similar manner to the selection processes occurring in the female reproductive tract. The results of these preliminary studies are encouraging, and larger trials are underway to evaluate using these methods in the field. Stallion semen could be processed in 100 mL glass tubes without a loss of sperm quality, and similarly, boar semen could be processed in 200 mL and 500 mL tubes without losing sperm quality. The objective of the present study was to scale up the SLC method for both stallion and boar semen. However, where stallion or boar semen is to be used for AI, larger volumes of semen have to be processed than for other species, thus limiting the effectiveness of the original technique. Single-Layer centrifugation has been used to improve the quality of sperm samples in several species.
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